Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters

  title={Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters},
  author={Geisa A. L. Gonçalves and Pedro H. Oliveira and Ana Gabriela Gomes and Kristala Jones Prather and Leslie A. Lewis and Duarte Miguel F. Prazeres and Gabriel A. Monteiro},
  journal={Applied Microbiology and Biotechnology},
Insertion specificity of mobile genetic elements is a rather complex aspect of DNA transposition, which, despite much progress towards its elucidation, still remains incompletely understood. We report here the results of a meta-analysis of IS2 target sites from genomic, phage, and plasmid DNA and find that newly acquired IS2 elements are consistently inserted around abrupt DNA compositional shifts, particularly in the form of switch sites of GC skew. The results presented in this study not only… 

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