Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease.

@article{Ryan2004EvidenceTP,
  title={Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease.},
  author={Kevin Ryan and Olga Calvo and James L Manley},
  journal={RNA},
  year={2004},
  volume={10 4},
  pages={565-73}
}
Generation of the polyadenylated 3' end of an mRNA requires an endonucleolytic cleavage followed by synthesis of the poly(A) tail. Despite the seeming simplicity of the reaction, more than a dozen polypeptides are required, and nearly all appear to be necessary for the cleavage reaction. Because of this complexity, the identity of the endonuclease has remained a mystery. Here we present evidence that a component of the cleavage-polyadenylation specificity factor CPSF-73 is the long-sought… CONTINUE READING