Evidence that phospholipase C from the sperm is not responsible for initiating Ca(2+) release at fertilization in mouse eggs.

@article{Mehlmann2001EvidenceTP,
  title={Evidence that phospholipase C from the sperm is not responsible for initiating Ca(2+) release at fertilization in mouse eggs.},
  author={Lisa M. Mehlmann and Abanti Chattopadhyay and Graham F Carpenter and Laurinda A Jaffe},
  journal={Developmental biology},
  year={2001},
  volume={236 2},
  pages={492-501}
}
Release of Ca(2+) from intracellular stores at fertilization of mammalian eggs is mediated by inositol 1,4,5-trisphosphate (IP3), but the mechanism by which the sperm initiates IP3 production is not yet understood. We tested the hypothesis that phospholipase C (PLC) activity introduced into the mouse egg as a consequence of sperm-egg fusion is responsible for causing Ca(2+) release. We demonstrated that microinjecting purified, recombinant PLCgamma1 protein into mouse eggs caused Ca(2… CONTINUE READING

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