Evidence that endo-1,4-beta-glucanases act on cellulose in suspension-cultured poplar cells.

@article{Ohmiya2000EvidenceTE,
  title={Evidence that endo-1,4-beta-glucanases act on cellulose in suspension-cultured poplar cells.},
  author={Yasunori Ohmiya and Masahiro Samejima and Mitsunori Shiroishi and Yoshihiko Amano and Takahisa Kanda and Fukumi Sakai and Takahisa Hayashi},
  journal={The Plant journal : for cell and molecular biology},
  year={2000},
  volume={24 2},
  pages={147-58}
}
Suspension-cultured poplar (Populus alba) cells produce two distinct endo-1,4-beta-glucanases, one of which is released in the extracellular culture medium and the other localized in their walls. Two cDNA clones, PopCel1 and PopCel2, isolated from a poplar cDNA library, encode the extracellular and the wall-bound endo-1, 4-beta-glucanases, respectively, based upon deduced amino acid sequences. The products of these two genes contained domains conserved in endo-1,4-beta-glucanase (family 9) and… CONTINUE READING
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