Evidence that a copper-metallothionein complex is responsible for fluorescence in acid-secreting cells of the Drosophila stomach

@article{McNulty2001EvidenceTA,
  title={Evidence that a copper-metallothionein complex is responsible for fluorescence in acid-secreting cells of the Drosophila stomach},
  author={Megan M. McNulty and Michael C Puljung and Greg Jefford and Ronald R. Dubreuil},
  journal={Cell and Tissue Research},
  year={2001},
  volume={304},
  pages={383-389}
}
Copper cells were originally identified in Drosophila midgut epithelium by their striking orange fluorescence in copper-fed larvae. Here, we examined copper cell fluorescence in light of the previous observations that (1) a similar fluorescent signal in yeast is produced by a complex between copper and metallothionein, and (2) metallothionein is expressed constitutively in the copper cell region and inducibly in other regions of the Drosophila midgut. Pulse-feeding experiments with 1 mM CuCl2… CONTINUE READING

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