Evidence that a 27-residue sequence is the actin-binding site of ABP-120.

  title={Evidence that a 27-residue sequence is the actin-binding site of ABP-120.},
  author={Anne R. Bresnick and Paul A. Janmey and John S Condeelis},
  journal={The Journal of biological chemistry},
  volume={266 20},
Proteolysis experiments of ABP-120 from Dictyostelium discoideum have previously demonstrated that removal of residues 89-115 from a tryptic peptide which retains actin binding activity, abolishes actin binding (Bresnick, A. R., Warren, V., and Condeelis, J. (1990) J. Biol. Chem. 265, 9236-9240). Antibodies made against a synthetic peptide of this 27-amino acid sequence (27-mer) specifically immunoprecipitate native ABP-120 from Dictyostelium high speed supernatants, demonstrating that the 27… CONTINUE READING

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