Evidence that DNase I hypersensitive site 5 of the human beta-globin locus control region functions as a chromosomal insulator in transgenic mice.

@article{Li2002EvidenceTD,
  title={Evidence that DNase I hypersensitive site 5 of the human beta-globin locus control region functions as a chromosomal insulator in transgenic mice.},
  author={Qiliang Li and Miaohua Zhang and Hemei Han and Alex D. Rohde and G. Stamatoyannopoulos},
  journal={Nucleic acids research},
  year={2002},
  volume={30 11},
  pages={2484-91}
}
We have previously reported that DNase I hypersensitive site 5 (5'HS5) of the human beta-globin locus control region functions as a chromatin insulator in stable transfection assays. In this report we show that a 3.2 kb DNA fragment containing the entire 5'HS5 region can protect a position-sensitive (A)gamma-globin gene against position effects in transgenic mice. Bracketing is required for function of 5'HS5 as an insulator. The 5'HS5 insulator operates in adult as well as in embryonic murine… CONTINUE READING

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