Evidence that BmTXKβ–BmKCT cDNA from Chinese scorpion Buthus martensii Karsch is an artifact generated in the reverse transcription process

  title={Evidence that BmTXK$\beta$–BmKCT cDNA from Chinese scorpion Buthus martensii Karsch is an artifact generated in the reverse transcription process},
  author={Xianchun Zeng and San-Xia Wang},
  journal={FEBS Letters},
cDNA is a man-made copy of mRNA. There are risks of producing artifactual copies of natural mRNAs in the process of in vitro cDNA synthesis. For example, cDNA synthesis can be primed either from a poly(A) tail of mRNA or from an A-rich internal region. Some artifacts could arise from a GCstem skipping mechanism in the reverse transcription reaction. Reverse transcription is also known to be an error-prone process and thus might generate mutations in the cDNA. Moreover, it has been shown that… Expand

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