Evidence of antiangiogenic and antimetastatic activities of the recombinant disintegrin domain of metargidin.

@article{TrochonJoseph2004EvidenceOA,
  title={Evidence of antiangiogenic and antimetastatic activities of the recombinant disintegrin domain of metargidin.},
  author={V{\'e}ronique Trochon-Joseph and Dominique Martel-Renoir and Lluis M. Mir and Annick Thoma{\"i}dis and Paule Opolon and Elisabeth Connault and Hong Li and Christophe Grenet and Françoise Fauvel-Laf{\`e}ve and Jeannette Soria and Chantal Legrand and C. I. M. A. Arturo Soria and M. Perricaudet and He Min Lu},
  journal={Cancer research},
  year={2004},
  volume={64 6},
  pages={2062-9}
}
Metargidin, a transmembrane protein of the adamalysin family, and integrins, e.g., alpha5beta1 and alphav, are preferentially expressed on endothelial cells on angiogenesis. Furthermore, metargidin interacts with these integrins via its disintegrin domain. In this study, recombinant human disintegrin domain (RDD) was produced in Escherichia coli by subcloning its cDNA into the pGEX-2T vector, and the effect of purified RDD on different steps of angiogenesis was evaluated. At concentrations of 2… CONTINUE READING