Evidence of a trimolecular complex involving LPS, LPS binding protein and soluble CD14 as an effector of LPS response

  title={Evidence of a trimolecular complex involving LPS, LPS binding protein and soluble CD14 as an effector of LPS response},
  author={C. Thomas and M. Kapoor and S. Sharma and H. Bausinger and Umit Zyilan and D. Lipsker and D. Hanau and A. Surolia},
  journal={FEBS Letters},
  • C. Thomas, M. Kapoor, +5 authors A. Surolia
  • Published 2002
  • Medicine, Chemistry
  • FEBS Letters
  • The kinetics of the interaction of lipopolysaccharide (LPS), lipopolysaccharide binding protein (LBP) and CD14 was studied using surface plasmon resonance. The association and dissociation rate constants for the binding of LPS and rsCD14 were 2.9×104 M−1 s−1 and 0.07 s−1 respectively, yielding a binding constant of 4.2×105 M−1. Significantly, the presence of LBP increased not only the association rate but also the association constant for the interaction between LPS and CD14 by three orders of… CONTINUE READING
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