Evidence linking the Pseudomonas oleovorans alkane omega-hydroxylase, an integral membrane diiron enzyme, and the fatty acid desaturase family.

@article{Shanklin2003EvidenceLT,
  title={Evidence linking the Pseudomonas oleovorans alkane omega-hydroxylase, an integral membrane diiron enzyme, and the fatty acid desaturase family.},
  author={John Shanklin and Edward J Whittle},
  journal={FEBS letters},
  year={2003},
  volume={545 2-3},
  pages={188-92}
}
Pseudomonas oleovorans alkane omega-hydroxylase (AlkB) is an integral membrane diiron enzyme that shares a requirement for iron and oxygen for activity in a manner similar to that of the non-heme integral membrane desaturases, epoxidases, acetylenases, conjugases, ketolases, decarbonylase and methyl oxidases. No overall sequence similarity is detected between AlkB and these desaturase-like enzymes by computer algorithms; however, they do contain a series of histidine residues in a similar… CONTINUE READING

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To test whether these conserved histidine residues are functionally equivalent to those of the desaturase - like enzymes we used scanning alanine mutagenesis to test if they are essential for activity of AlkB.
To test whether these conserved histidine residues are functionally equivalent to those of the desaturase - like enzymes we used scanning alanine mutagenesis to test if they are essential for activity of AlkB.
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