Evidence for two high-affinity bradykinin binding sites in the guinea-pig lung.

@article{Trifilieff1991EvidenceFT,
  title={Evidence for two high-affinity bradykinin binding sites in the guinea-pig lung.},
  author={A Trifilieff and E B Haddad and Yves Landry and J P Gies},
  journal={European journal of pharmacology},
  year={1991},
  volume={207 2},
  pages={129-34}
}
We examined the binding of [3H]bradykinin ([3H]BK) to the guinea-pig lung which was saturable. Scatchard analysis indicated the existence of two binding sites, one with a high affinity (KD = 15 pM) and one with a low one (KD = 570 pM) with maximum number of binding sites of 12 and 45 fmol/mg protein, respectively. Kinetic studies confirmed the presence of these two types of binding sites and their affinity ranges. Neither the B1 agonist des-Arg9-BK, nor the B1 antagonist des-Arg9-[Leu8]BK… CONTINUE READING

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