Evidence for two different Rieske iron-sulfur proteins in the cytochrome bf complex of spinach chloroplast.

@article{Yu1994EvidenceFT,
  title={Evidence for two different Rieske iron-sulfur proteins in the cytochrome bf complex of spinach chloroplast.},
  author={Shyr Yu and Elzbieta Romanowska and Z T Xue and Per A. Albertsson},
  journal={Biochimica et biophysica acta},
  year={1994},
  volume={1185 2},
  pages={239-42}
}
Spinach thylakoids, grana vesicles, stroma lamellae vesicles and also isolated cytochrome bf complex, were analysed by two-dimensional polyacrylamide gel electrophoresis employing isoelectric focusing in the first dimension and sodium dodecyl-sulfate polyacrylamide gel electrophoresis in the second dimension. About 100 thylakoid membrane proteins were resolved. In all cases the Rieske FeS protein separated into two polypeptide spots having the isoelectric points of 5.1 and 5.4, respectively… CONTINUE READING