Evidence for the simultaneous translocation of muscarinic acetylcholine receptor and G protein by carbachol.

@article{Ho1991EvidenceFT,
  title={Evidence for the simultaneous translocation of muscarinic acetylcholine receptor and G protein by carbachol.},
  author={A. Ho and Y. Zhang and R. Duffield and G. Zheng},
  journal={Cellular signalling},
  year={1991},
  volume={3 6},
  pages={
          587-98
        }
}
Interactions between guanine nucleotide regulatory proteins (G proteins) and muscarinic acetylcholine receptors (mAChRs) were studied in vivo following carbachol treatment. Rat brain homogenates were separated by high speed ultracentrifugation into heavy and light membrane and 300,000 g supernate fractions. The G proteins were partially purified by Sephadex-G200 and heptylamine-Sepharose and the mAChRs by (3,2'-aminobenzhydryloxy)-tropane-(ABT)-affinity chromatographies. Radioligand binding… Expand
9 Citations
Distinct mechanisms for the endocytosis of muscarinic receptors and Gq/11.
CHAPTER 7 – Receptor Regulation

References

SHOWING 1-9 OF 9 REFERENCES
Reconstitution of resolved muscarinic cholinergic receptors with purified GTP-binding proteins.
Purification of the muscarinic acetylcholine receptor from porcine brain.
  • K. Haga, T. Haga
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • 1985
Purification of the major GTP-binding proteins from human placental membranes.
Phosphorylation of brain muscarinic receptor: evidence of receptor regulation.