Evidence for the presence of two active forms of cytosolic 3,5,3'-triiodo-L-thyronine (T3)-binding protein (CTBP) in rat kidney. Specialized functions of two CTBPs in intracellular T3 translocation.

@article{Hashizume1989EvidenceFT,
  title={Evidence for the presence of two active forms of cytosolic 3,5,3'-triiodo-L-thyronine (T3)-binding protein (CTBP) in rat kidney. Specialized functions of two CTBPs in intracellular T3 translocation.},
  author={Kiyoshi Hashizume and Takahide Miyamoto and Kazuo Ichikawa and Kazumi Yamauchi and Akihiro Sakurai and Hiromichi Ohtsuka and Makio Kobayashi and Yutaka Nishii and Tambur{\'u}s Nayara Yamada},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 9},
  pages={
          4864-71
        }
}
Cytosolic NADPH-dependent 3,5,3'-triiodo-L-thyronine (T3)-binding protein (CTBP) purified from rat kidney was further characterized in its T3 binding and its interaction with nuclei. Pretreatment of the CTBP with NADP induced dithiothreitol (DTT)-dependent T3 binding. The DTT-dependent T3 binding was increased by NADP in a concentration-dependent manner, and the maximal binding was obtained by 0.1 microM NADP. Higher concentrations of NADP (more than 0.1 microM), however, reduced T3 binding… CONTINUE READING