Evidence for the presence of messenger ribonucleic acid in Allomyces macrogynus mitospores

  title={Evidence for the presence of messenger ribonucleic acid in Allomyces macrogynus mitospores},
  author={B. A. Smith and D D Burke},
  journal={Journal of Bacteriology},
  pages={535 - 541}
Sucrose density gradient analysis was used to show that polysomes were present in the mitospores of Allomyces macrogynus. Fifty percent of the spore monosomes were shown to be resistant to dissociation by 0.8 M KCl, indicating that messenger ribonucleic acid (mRNA) was bound to them. These polysomes and all the spore ribosomes were contained in the nuclear cap. Only 4S RNA could be demonstrated in the extra-cap fraction. Hybridization studies using 3H-labeled polydeoxythymidylic acid indicated… 
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