Evidence for the presence of β-subunit of hexosaminidase in a case of Sandhoff disease using a blotting technique

@article{Gautron2004EvidenceFT,
  title={Evidence for the presence of $\beta$-subunit of hexosaminidase in a case of Sandhoff disease using a blotting technique},
  author={S. Gautron and L. Poenaru and J. Boue and H. Puissant and J. Lisman and J. Dreyfus},
  journal={Human Genetics},
  year={2004},
  volume={63},
  pages={258-261}
}
SummaryHexosaminidases, lysosomal enzymes whose deficiency is responsible for several genetic disorders, exist as two major forms: form A, containing two types of subunits α and β; and form B, containing only β subunits.We have used a technique involving successively electrophoresis of denatured proteins, transfer (blotting) onto nitrocellulose, and labelling by appropriate antibodies raised against the dissociated forms of hexosaminidases A and B. This technique allows the detection of α and… Expand
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