Evidence for the involvement of tetrahydrofolate in the demethylation of nicotine by Nicotiana plumbaginifolia cell-suspension cultures

@article{Mesnard2002EvidenceFT,
  title={Evidence for the involvement of tetrahydrofolate in the demethylation of nicotine by Nicotiana plumbaginifolia cell-suspension cultures},
  author={François Mesnard and Albrecht Roscher and Andrew P. Garlick and Sandrine Girard and Evelyne Baguet and Randolf R. Arroo and Jacques Lebreton and Richard J. Robins and George R. Ratcliffe},
  journal={Planta},
  year={2002},
  volume={214},
  pages={911-919}
}
The conversion of nicotine to nornicotine by Nicotiana plumbaginifolia Viv. cells was investigated by analysing the redistribution of label during feeding experiments with (R,S)-[2H-methyl]nicotine, (R,S)-[13C-methyl]nicotine and (R,S)-[14C-methyl]nicotine, and the results show that the N-methyl group of nicotine can be recycled into primary metabolism. Nuclear magnetic resonance (NMR) analysis of ethanolic extracts of cells grown in the presence of (R,S)-[13C-methyl]nicotine, using 1H-13C… CONTINUE READING

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