Evidence for the involvement of arginyl residue at the active site of penicillin G acylase from Kluyvera citrophila

@article{Kumar2004EvidenceFT,
  title={Evidence for the involvement of arginyl residue at the active site of penicillin G acylase from Kluyvera citrophila},
  author={Rajnish Kumar and Cheravakkattu Gopalan Suresh and Archana Vishnu Pundle and Asmita Prabhune},
  journal={Biotechnology Letters},
  year={2004},
  volume={26},
  pages={1601-1606}
}
Penicillin G acylase (PGA) is used for the commercial production of semi-synthetic penicillins. It hydrolyses the amide bond in penicillin producing 6-aminopenicillanic acid and phenylacetate. 6-Aminopenicillanic acid, having the β-lactam nucleus, is the parent compound for all semi-synthetic penicillins. Penicillin G acylase from Kluyvera citrophila was purified and chemically modified to identify the role of arginine in catalysis. Modification with 20 mm phenylglyoxal and 50 mm 2,3… CONTINUE READING
1 Citations
16 References
Similar Papers

Citations

Publications citing this paper.

References

Publications referenced by this paper.
Showing 1-10 of 16 references

Computational analysis of the amino acid subsite of PGA explain the influence of amine structure on enantioselectivity

  • A Basso, P Braiuca, S Clementi, C Ebert, L Gardossi, P Linda
  • J. Mol. Catal. B: Enzym
  • 2002
1 Excerpt

Penicillin acylase in the industrial production of β-lactam antibiotics

  • A Bruggink, ER Roos, E De Vroom
  • Org. Proc. Res. Dev
  • 1998
2 Excerpts

Penicillin acylase has a single aminoacid catalytic center

  • HJ Duggleby, SP Tolley, CP Hill, EJ Dodson, G Dodson, Moody PCE
  • Nature
  • 1995
1 Excerpt

Evaluation and determination of 6- aminopenicillinic acid by p-dimethylbenzaldehyde

  • GJ Shewale, KK Kumar, GR Amberkar
  • Biotechnol. Tech
  • 1987
2 Excerpts

Similar Papers

Loading similar papers…