Evidence for the glyoxyiate cycle in human liver

@article{Davis1992EvidenceFT,
  title={Evidence for the glyoxyiate cycle in human liver},
  author={Walter L. Davis and David B. P. Goodman},
  journal={The Anatomical Record},
  year={1992},
  volume={234}
}
The enzymatic activities unique to the glyoxylate cycle of higher plants and certain lower invertebrates, isocitrate lyase and malate synthase, have been demonstrated in homogenates prepared from human liver. Human liver can also carry out cyanide‐insensitive fatty acid oxidation from palmitate. Utilizing light microscopic immunocytochemistry with an antibody produced against Euglena malate synthase, this enzyme localizes in numerous ovoid granules in human hepatocytes. Also… 
The absence of glyoxylate cycle enzymes in rodent and embryonic chick liver.
  • R. Holmes
  • Biology, Medicine
    Biochimica et biophysica acta
  • 1993
TLDR
The results indicate that when assaying enzyme activities in crude tissue homogenates specific methods for the identification of end products are required.
Human liver glycolate oxidase : gene identification and protein studies
TLDR
To identify human GO, characterise the kinetics and substrate specificity of the enzyme and establish methods for the analysis of relevant metabolic pathways in vitro, HPLC methods were developed for the separation and quantitation of glyoxylate, hydroxypyruvate and pyruvates, enabling analysis of metabolites produced by GO and neighboring enzymes in the metabolic pathway.
Comparative Analysis of Glyoxylate Cycle Key Enzyme Isocitrate Lyase from Organisms of Different Systematic Groups
TLDR
The obtained data raise the question about the pathway of evolution of genes of the glyoxylate cycle key enzymes in cells of prokaryotes, plants, fungi, and nematodes in response to arising demands in carbohydrates.
CLYBL is a polymorphic human enzyme with malate synthase and β-methylmalate synthase activity.
TLDR
Using comparative genomics, it is shown that CLYBL is strongly co-expressed with and co-evolved specifically with other components of the mitochondrial B12 pathway, and it is confirmed that the premature stop polymorphism in CLyBL leads to a loss of protein expression.
Gluconeogenesis and the peroxisome
  • C. Masters
  • Biology, Medicine
    Molecular and Cellular Biochemistry
  • 2004
TLDR
The capabilities of peroxisomal involvement in the gluconeogenetic processes of vertebrate animals are reviewed and it is demonstrated that the participation of this organelle affords the potential of alternative pathways for the conversion of triacylglycerols to glucose, and for the Conversion of amino acids and lactate to carbohydrate.
Induction of a Peroxisomal Malate Dehydrogenase Isoform in Liver of Starved Rats
TLDR
It is suggested that the newly expressed isoform of MDH operates in the glyoxylate cycle of liver peroxisomes of food-starved animals.
Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes
TLDR
The hydrodynamic transfer of two glyoxylate cycle enzymes could accomplish the shift of using fat for the synthesis of glucose in mice to induce fat utilization for glucose synthesis, which might be eventually useful to reduce body fat depots in situations of obesity and diabetes.
Identification and characterization of cytosolic malate dehydrogenase from the liver fluke Fasciola gigantica
TLDR
The cytosolic malate dehydrogenase in F. gigantica is identified and the enzyme biochemically and structurally characterized and the structural and functional properties of MDH are understood to better understand the roles of this enzyme in the biochemistry of the parasite.
Structure of the isocitrate lyase gene of cucumber and its transcriptional regulation in higher plants
TLDR
F fluorometric and histochemical analysis of the transformants indicated that, in vivo, both promoter fragments faithfully directed the temporal regulation of icl gene expression during postgerminative growth, illustrating that there are both developmental and metabolic controls acting on the icl genes of cucumber.
Introduction and expression of the bacterial glyoxylate cycle genes in transgenic mice
TLDR
Two bacterial genes, encoding ICL and MS, were modified in order to introduce them into the mouse germ line and enzyme activities were detected in the liver and intestine at levels up to 25% of those measured in fully derepressedEscherichia coli cells.
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