Evidence for the expression of four myelin basic protein variants in the developing human spinal cord through cDNA cloning

@article{Roth1987EvidenceFT,
  title={Evidence for the expression of four myelin basic protein variants in the developing human spinal cord through cDNA cloning},
  author={H. John Roth and Kathryn E. Kronquist and Nicole Kerlero de Rosbo and Barbara F. Crandall and Anthony T. Campagnoni},
  journal={Journal of Neuroscience Research},
  year={1987},
  volume={17}
}
Four human myelin basic protein (MBP) variants with molecular masses of 21.5, 20.2, 18.5, and 17.3 kilodaltons (kDa) have been identified in the developing human spinal cord and their structures determined through an analysis of cDNA clones of their mRNAs. The 20.2‐kDa MBP mRNA encoded a novel MBP variant, the structure of which has not been reported in any species. Its amino acid sequence was identical with that of the 21.5‐kDa MBP except for a deletion of 11 amino acid residues encoded by… 
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Expression of a Novel Transcript of the Myelin Basic Protein Gene
TLDR
The results suggest that the structure of the MBP gene is more complex than originally thought, containing at least two more exons and there appears to be at least one moreMBP gene promoter that directs the synthesis of a subset of MBP mRNAs with a unique 5′‐untranslated region.
Expression of myelin proteins in the developing human spinal cord: Cloning and sequencing of human proteolipid protein cDNA
TLDR
A full‐length clone for the human proteolipid protein (PLP) was isolated from a cDNA library constructed from poly (A)+ RNA isolated from fetal spinal cords obtained at 15–24 weeks of conceptional age, and the deduced amino acid sequence was found to be identical with that of rat PLP.
Purification of immunologically active recombinant 21.5 kDa isoform of human myelin basic protein.
Genetic alterations in the mouse myelin basic proteins result in a range of dysmyelinating disorders
  • E. Jacobs
  • Biology
    Journal of the Neurological Sciences
  • 2005
Organization and expression of the human myelin basic protein gene
TLDR
The human brain contains four isoforms of myelin basic protein (MBP), previously identified by cDNA cloning, and isolated and characterized genomic clones encoding the human MBP gene, which consists of seven exons.
Expression of the myelin basic protein gene locus in neurons and oligodendrocytes in the human fetal central nervous system
TLDR
By using reverse transcriptase‐polymerase chain reaction cloning, eight new members of the Golli gene family of transcripts expressed in the human CNS are identified and it is indicated that their function is quite different from that of the MBPs to which they are closely related.
Protein factors and 5' flanking sequences involved in the expression of the mouse myelin basic protein gene
TLDR
This project was initiated by the isolation of the putative promoter region of the mouse myelin basic protein (MBP) gene and the presence of protein factors specifically binding to the region -609 to -577 was demonstrated by electrophoretic mobility shift assay (EMSA).
Developmental Study of Myelin Basic Protein Variants in Various Regions of Pig Nervous System
TLDR
Results suggest that in the pig NS, the expression of MBP variants may be regulated both regionally and developmentally.
Molecular Biology of Myelin Proteins from the Central Nervous System
TLDR
Most of the dysmyelinating mutants, including those in which the genetic defect is established, appear to exhibit pleiotropy with respect to the expression of other myelin protein genes, and their structures have been established through an analysis of the cDNA clones encoding them.
Myelin Basic Protein Gene
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References

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TLDR
Southern blotting studies of human genomic DNA reveal a simple pattern consistent with a single human MBP gene, and the three MBP mRNAs are likely to arise from alternative splicing of a primary humanMBP transcript.
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TLDR
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TLDR
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Alternative splicing accounts for the four forms of myelin basic protein
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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