Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing

@article{Pikielny1983EvidenceFT,
  title={Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing},
  author={Claudio W. Pikielny and John L. Teem and Michael Rosbash},
  journal={Cell},
  year={1983},
  volume={34},
  pages={395-403}
}
Sequence comparison of the introns of two yeast genes (rp51A and rp51B) coding for the same ribosomal protein shows homology only in the last 50 bases of the intron. This region of the intron contains an internal conserved sequence (ICS) present near the 3' end of all sequenced yeast nuclear mRNA introns. Removal of a 29 bp sequence containing the ICS prevents splicing of an intron-containing hybrid gene. In cells containing the wild-type gene, we have detected RNA molecules that we suggest are… Expand
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References

SHOWING 1-10 OF 55 REFERENCES
Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts
TLDR
It is suggested that the intron-contained sequence contains the octanucleotide 5'-TACTAACA-3', which occurs 20 to 55 nucleotides upstream from the 3' splice site in all split protein-coding nuclear genes from S. cerevisiae sequenced to date, and is an essential element of the yeast splicing mechanism, involved in the selection of splicing sites. Expand
Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast.
  • J. Teem, M. Rosbash
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1983
TLDR
The experiments suggest that an intron-containing beta-galactosidase gene can be used in a general way to study mRNA splicing, consistent with the notion that this mutation affects gene expression at the level of splicing. Expand
The structure of the gene coding for the phosphorylated ribosomal protein S10 in yeast.
TLDR
The split character of one of these ribosomal protein genes, viz. the gene coding for the major phosphorylated small-subunit protein S10, was further established by sequence analysis and a striking homology between both proteins was revealed. Expand
Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.
TLDR
Comparison of the Drosophila U1 sequence with vertebrate U1 sequences allows a particular secondary structure model to be preferred over others and suggest specific modifications of the model detailing molecular interactions between U1 RNA and hnRNA during the splicing reaction. Expand
Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene.
TLDR
The 5' termini of yeast CYC1 RNA molecules have been mapped, by nuclease S1 digestion of mRNA, to seven locations from 29 to 93 base pairs upstream from the initiating ATG codon to measure the capacity of enzymatically produced DNA deletions to form the normal set of RNAs. Expand
Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.
  • D. Gallwitz, I. Sures
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1980
TLDR
The complete nucleotide sequence of the actin gene from Saccharomyces cerevisiae has been determined and its primary structure, especially the NH2-terminal third of the protein, is highly conserved during evolution. Expand
Construction of a yeast actin gene intron deletion mutant that is defective in splicing and leads to the accumulation of precursor RNA in transformed yeast cells.
  • D. Gallwitz
  • Medicine, Biology
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1982
TLDR
Several intron deletion mutants were constructed to define sequences at the 5' splice junction that are required for RNA splicing by using nuclease BAL-31, and extensive parts of the intron can be removed without affecting correct splicing. Expand
Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena
TLDR
It is proposed that the IVS portion of the RNA has several enzyme-like properties that enable it to break and reform phosphodiester bonds and that enzymes, small nuclear RNAs and folding of the pre-rRNA into an RNP are unnecessary for these reactions. Expand
The U1 small nuclear RNA-protein complex selectively binds a 5′ splice site in vitro
The ability of purified U1 small nuclear RNA-protein complexes (U1 snRNPs) to bind in vitro to two RNAs transcribed from recombinant DNA clones by bacteriophage T7 RNA polymerase has been studied. AExpand
Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure.
TLDR
A previously unsuspected wealth of evolutionarily conserved sequences and secondary structures was uncovered, and at least seven at least of the available sequences may be folded up into elaborate secondary structure models, the cores of which are nearly identical. Expand
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