Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing

@article{Pikielny1983EvidenceFT,
  title={Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing},
  author={C. Pikielny and J. Teem and M. Rosbash},
  journal={Cell},
  year={1983},
  volume={34},
  pages={395-403}
}
  • C. Pikielny, J. Teem, M. Rosbash
  • Published 1983
  • Biology, Medicine
  • Cell
  • Sequence comparison of the introns of two yeast genes (rp51A and rp51B) coding for the same ribosomal protein shows homology only in the last 50 bases of the intron. This region of the intron contains an internal conserved sequence (ICS) present near the 3' end of all sequenced yeast nuclear mRNA introns. Removal of a 29 bp sequence containing the ICS prevents splicing of an intron-containing hybrid gene. In cells containing the wild-type gene, we have detected RNA molecules that we suggest are… CONTINUE READING
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