Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing

@article{Pikielny1983EvidenceFT,
  title={Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing},
  author={Claudio W. Pikielny and John L. Teem and Michael Rosbash},
  journal={Cell},
  year={1983},
  volume={34},
  pages={395-403}
}

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References

SHOWING 1-10 OF 59 REFERENCES

Expression of a beta-galactosidase gene containing the ribosomal protein 51 intron is sensitive to the rna2 mutation of yeast.

  • J. TeemM. Rosbash
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1983
The experiments suggest that an intron-containing beta-galactosidase gene can be used in a general way to study mRNA splicing, consistent with the notion that this mutation affects gene expression at the level of splicing.

The structure of the gene coding for the phosphorylated ribosomal protein S10 in yeast.

The split character of one of these ribosomal protein genes, viz. the gene coding for the major phosphorylated small-subunit protein S10, was further established by sequence analysis and a striking homology between both proteins was revealed.

Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.

Comparison of the Drosophila U1 sequence with vertebrate U1 sequences allows a particular secondary structure model to be preferred over others and suggest specific modifications of the model detailing molecular interactions between U1 RNA and hnRNA during the splicing reaction.

Deletion mapping of sequences essential for in vivo transcription of the iso-1-cytochrome c gene.

The 5' termini of yeast CYC1 RNA molecules have been mapped, by nuclease S1 digestion of mRNA, to seven locations from 29 to 93 base pairs upstream from the initiating ATG codon to measure the capacity of enzymatically produced DNA deletions to form the normal set of RNAs.

Structure of a split yeast gene: complete nucleotide sequence of the actin gene in Saccharomyces cerevisiae.

  • D. GallwitzI. Sures
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1980
The complete nucleotide sequence of the actin gene from Saccharomyces cerevisiae has been determined and its primary structure, especially the NH2-terminal third of the protein, is highly conserved during evolution.

Construction of a yeast actin gene intron deletion mutant that is defective in splicing and leads to the accumulation of precursor RNA in transformed yeast cells.

  • D. Gallwitz
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1982
Several intron deletion mutants were constructed to define sequences at the 5' splice junction that are required for RNA splicing by using nuclease BAL-31, and extensive parts of the intron can be removed without affecting correct splicing.
...