Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing

@article{Pikielny1983EvidenceFT,
  title={Evidence for the biochemical role of an internal sequence in yeast nuclear mRNA introns: Implications for U1 RNA and metazoan mRNA splicing},
  author={Claudio W. Pikielny and John L. Teem and Michael Rosbash},
  journal={Cell},
  year={1983},
  volume={34},
  pages={395-403}
}
Sequence comparison of the introns of two yeast genes (rp51A and rp51B) coding for the same ribosomal protein shows homology only in the last 50 bases of the intron. This region of the intron contains an internal conserved sequence (ICS) present near the 3' end of all sequenced yeast nuclear mRNA introns. Removal of a 29 bp sequence containing the ICS prevents splicing of an intron-containing hybrid gene. In cells containing the wild-type gene, we have detected RNA molecules that we suggest are… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 25 CITATIONS

Human branch point consensus sequence is yUnAy

  • Nucleic acids research
  • 2008
VIEW 1 EXCERPT
CITES BACKGROUND

References

Publications referenced by this paper.
SHOWING 1-10 OF 43 REFERENCES

A catalogue of splice junction sequences.

  • Nucleic acids research
  • 1982
VIEW 6 EXCERPTS
HIGHLY INFLUENTIAL

A mechanism for RNA splicing.

  • Proceedings of the National Academy of Sciences of the United States of America
  • 1980
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

Abnormal expression of chromosomal rabbit beta-globin in Sacchammyces cerevisiae. Nature

J. D. Beggs, J. van der Berg, A. van Ooyen, C. Weissman
  • Ann. Rev. Biochem
  • 1980
VIEW 3 EXCERPTS
HIGHLY INFLUENTIAL

Evidence for an introncontained sequence required for the splicing of yeast RNA polymerase II transcripts

C. J.. Nellen Langford, J. W..Niessing., D. Gallwltz
  • Cell
  • 1983
VIEW 2 EXCERPTS

Signals for the splicing of messenger L RNA transcripts

S. M. Mount, A Steitz.J.
  • In Methods of DNA and RNA Sequencing,
  • 1983