Evidence for the Presence of Noradrenaline in Submicroscopic Structures of Adrenergic Axons

  title={Evidence for the Presence of Noradrenaline in Submicroscopic Structures of Adrenergic Axons},
  author={Ulf S. von Euler and N. -{\AA}. Hillarp},
THE presence of sympathomimetic activity in adrenergic nerve tissue has been demonstrated by several authors1. The active substance later was shown to possess the characteristic biological and chemical properties of noradrenaline2. A comparison of the relative contents of noradrenaline in splenic nerves (10–20 µgm. per gm.) and the spleen (2–4 µgm. per gm.) suggests that the transmitter is accumulated in the terminal parts of the nerves. Certain substances, such as acids, detergents and… 
Effect of Noradrenaline on Phosphatase Activity in Synaptic Membrane of the Rat Brain
In vitro investigations revealed phosphatidyl-serine to be the only major lipid among phospholipids or glycolipids free from sialic acid of the CNS which binds the noradrenaline, and in vitro investigations stimulated the search for a possible function of phosphatIDyl-Serine in the synaptic endings.
The importance of axoplasmic transport of amine granules for the functions of adrenergic neurons.
Results obtained with and without reserpine pre-treatment indicate that the new amine granules may be more active in storing 3H-NA than the older granules, and also the release of the transmitter may occur predominatly from the new granules.
Synthesis, Uptake and Storage of Catecholamines in Adrenergic Nerves, The Effect of Drugs
Detailed studies on isolated particles have shown that although similarities occur, important differences between the properties of the chromaffin cell particles and those in the adrenergic nerves exist.
The regulation of the noradrenergic neuron.
The Presence of the Adrenergic Nenrotransmitter in Intraaxonal Structures.
It is concluded that noradrenaline is present in high concentration in disperse structures in the adrenergic axones in cell-free homogenates of adrenergic nerves by high speed centrifugation.
Release of Catecholamines
The cornerstone of the neurohumoral theory of synaptic transmission is the ability of nerves to release an active neurotransmitter on stimulation, and classical experiments in neuropharmacology were the application of sensitive and quite specific bioassays to demonstrate the release of active hormone on stimulation of a peripheral nerve.
Axoplasmic Transport of 14C-Noradrenaline and Protein in Splenic Nerves
Because the calculation assumes that no noradrenaline is synthesized, lost to or taken up from the circulation in the movement of the storage granules down the axon and at the site of constriction, an alternative method, labelling both nor adrenaline and protein with isotopes, was used on the cat splenic nerves.