Evidence for functional heterogeneity among the catalytic sites of the bovine heart mitochondrial F1-ATPase.

@article{Bullough1987EvidenceFF,
  title={Evidence for functional heterogeneity among the catalytic sites of the bovine heart mitochondrial F1-ATPase.},
  author={David A. Bullough and J G Verburg and Masasuke Yoshida and William S. Allison},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 24},
  pages={11675-83}
}
The characteristics of ATP hydrolysis at a single catalytic site of the bovine heart F1-ATPase (MF1) as originally described by Grubmeyer et al. (Grubmeyer, C., Cross, R.L., and Penefsky, H.S. (1982) J. Biol. Chem. 257, 12092-12100) were compared with those of various chemically modified preparations of MF1 in which the steady state activity was severely attenuated. Although it was not necessary to age our preparations of native MF1 in the presence of 2 mM Pi to observe the same characteristics… CONTINUE READING