Evidence for early recruitment of granulocyte precursors during high-dose methotrexate infusions in mice.

@article{Pinedo1976EvidenceFE,
  title={Evidence for early recruitment of granulocyte precursors during high-dose methotrexate infusions in mice.},
  author={Herbert M. Pinedo and Bruce Allan Chabner and Daniel S. Zaharko and Joan M. C. Bull},
  journal={Blood},
  year={1976},
  volume={48 2},
  pages={301-7}
}
The effects of constant exposure to high concentrations of methotrexate in vivo on the committed stem cell (CFU-C) were studied by in vitro culture of mouse bone marrow. Bone marrow samples were obstained from animals receiving a continuous infusion, and were cultured in a methotrexate-free semisolid gel system. The effects of methotrexate infusion on the pluripotent stem cell population (CFU-S) were studied as well. Constant exposure to 10(-5) M methotrexate produced a rapid decrease in total… CONTINUE READING