Evidence for cytoskeletal changes secondary to plasma membrane functional alterations in the in vitro cell response to Clostridium perfringens epsilon-toxin.

@article{Donelli2003EvidenceFC,
  title={Evidence for cytoskeletal changes secondary to plasma membrane functional alterations in the in vitro cell response to Clostridium perfringens epsilon-toxin.},
  author={Gianfranco Donelli and Carla Fiorentini and P. Matarrese and Loredana Falzano and Rita Cardines and Paola Mastrantonio and Dean W Payne and Richard W. Titball},
  journal={Comparative immunology, microbiology and infectious diseases},
  year={2003},
  volume={26 3},
  pages={145-56}
}
To investigate the mode of action of Clostridium perfringens epsilon-toxin, MDCK cells were treated with purified toxin and incubated at 37 degrees C for up to 24h. Exposure to epsilon-toxin caused a time-dependent decrease in cell-cell and cell-substrate interactions. After 30min of treatment retraction of the cell body and the emission of filopodia were detectable in a number of cells. Longer exposure resulted in cell rounding and cell blebbing which reached a maximum after 5h of toxin… CONTINUE READING