Evidence for an internal promoter in the Escherichia coli threonine operon.


We constructed plasmids carrying the two first genes of the threonine operon from which the major promoter was deleted in vitro by digestion with BAL 31 nuclease. These plasmids continued to express the second gene (thrB) of the operon as judged by their ability to complement a threonine auxotroph. These data indicate that, in addition to the major promoter… (More)


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