Evidence for an essential histidine residue in the Neurospora crassa plasma membrane H+-ATPase.

Abstract

The Neurospora crassa plasma membrane H+-ATPase is rapidly inactivated in the presence of diethyl pyrocarbonate (DEP). The reaction is pseudo-first-order showing time- and concentration-dependent inactivation with a second-order rate constant of 385-420 M-1.min-1 at pH 6.9 and 25 degrees C. The difference spectrum of the native and modified enzyme has a… (More)

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Cite this paper

@article{Morjana1989EvidenceFA, title={Evidence for an essential histidine residue in the Neurospora crassa plasma membrane H+-ATPase.}, author={Nihmat Morjana and Gene A. Scarborough}, journal={Biochimica et biophysica acta}, year={1989}, volume={985 1}, pages={19-25} }