Evidence for an alternate model of human P-glycoprotein structure and biogenesis.

@article{Skach1993EvidenceFA,
  title={Evidence for an alternate model of human P-glycoprotein structure and biogenesis.},
  author={William R. Skach and M C Calayag and Vishwanath R. Lingappa},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 10},
  pages={6903-8}
}
We have studied the transmembrane topology of human P-glycoprotein (MDR1) using protein chimeras in Xenopus oocytes and full-length native protein in a cell-free translation system. We find both in vivo and in vitro, that the peptide region between putative transmembrane helices (TM) 8 and 9 resides in the endoplasmic reticulum lumen not in the cytosol as predicted. The topology of the carboxyl-terminal half of MDR1 therefore appears distinct from the homologous amino-terminal half in which the… CONTINUE READING

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