Evidence for a two-step mechanism involved in assembly of functional signal recognition particle receptor

@article{Andrews1989EvidenceFA,
  title={Evidence for a two-step mechanism involved in assembly of functional signal recognition particle receptor},
  author={David W. Andrews and Leander Lauffer and Peter J. Walter and Vishwanath R. Lingappa},
  journal={The Journal of Cell Biology},
  year={1989},
  volume={108},
  pages={797 - 810}
}
The signal recognition particle (SRP) and SRP receptor act sequentially to target nascent secretory proteins to the membrane of the ER. The SRP receptor consists of two subunits, SR alpha and SR beta, both tightly associated with the ER membrane. To examine the biogenesis of the SRP receptor we have developed a cell-free assay system that reconstitutes SR alpha membrane assembly and permits both anchoring and functional properties to be assayed independently. Our experiments reveal a mechanism… CONTINUE READING

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