Evidence for a tonoplast-associated form of sucrose synthase and its potential involvement in sucrose mobilization from the vacuole.

@article{Etxeberria2003EvidenceFA,
  title={Evidence for a tonoplast-associated form of sucrose synthase and its potential involvement in sucrose mobilization from the vacuole.},
  author={Ed Etxeberria and Pedro Olmos Gonzalez},
  journal={Journal of experimental botany},
  year={2003},
  volume={54 386},
  pages={1407-14}
}
The following work presents new evidence for a tonoplast localization of sucrose synthase and its functional role during the mobilization of vacuolar sucrose. Highly purified tonoplast vesicles were associated with significant SuSy levels as determined by immuno-recognition, enzymatic activity, and by-product measurements. Total tonoplast-bound SuSy was estimated to be approximately 7% of the total tissue activity. SuSy affinity to the tonoplast was confirmed by the lack of SuSy displacement by… CONTINUE READING

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