Evidence for a thermodynamically distinct Mg2+ ion associated with formation of an RNA tertiary structure.

@article{Leipply2011EvidenceFA,
  title={Evidence for a thermodynamically distinct Mg2+ ion associated with formation of an RNA tertiary structure.},
  author={Desirae Leipply and David E. Draper},
  journal={Journal of the American Chemical Society},
  year={2011},
  volume={133 34},
  pages={13397-405}
}
A folding strategy adopted by some RNAs is to chelate cations in pockets or cavities, where the ions neutralize charge from solvent-inaccessible phosphate. Although such buried Mg(2+)-RNA chelates could be responsible for a significant fraction of the Mg(2+)-dependent stabilization free energy of some RNA tertiary structures, direct measurements have not been feasible because of the difficulty of finding conditions under which the free energy of Mg(2+) chelation is uncoupled from RNA folding… CONTINUE READING