Evidence for a proton transfer network and a required persulfide-bond-forming cysteine residue in Ni-containing carbon monoxide dehydrogenases.

@article{Kim2004EvidenceFA,
  title={Evidence for a proton transfer network and a required persulfide-bond-forming cysteine residue in Ni-containing carbon monoxide dehydrogenases.},
  author={Eun Jin Kim and Jian Feng and Matthew R Bramlett and Paul A. Lindahl},
  journal={Biochemistry},
  year={2004},
  volume={43 19},
  pages={
          5728-34
        }
}
Carbon monoxide dehydrogenase from Moorella thermoacetica catalyzes the reversible oxidation of CO to CO(2) at a nickel-iron-sulfur active site called the C-cluster. Mutants of a proposed proton transfer pathway and of a cysteine residue recently found to form a persulfide bond with the C-cluster were characterized. Four semiconserved histidine residues were individually mutated to alanine. His116 and His122 were essential to catalysis, while His113 and His119 attenuated catalysis but were not… CONTINUE READING

References

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Carbon Monoxide Dehydrogenases and Acetyl-CoA Synthases: Light at the End of the Tunnel

  • P. A. Lindahl
  • Biochemistry
  • 2002
Highly Influential
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