Evidence for a gapped linear duplex DNA intermediate in the replicative cycle of human and simian spumaviruses.

@article{Kupiec1988EvidenceFA,
  title={Evidence for a gapped linear duplex DNA intermediate in the replicative cycle of human and simian spumaviruses.},
  author={Jean-Jacques Kupiec and Joelle Tobaly-Tapiero and Martine Canivet and Maud Santillana-Hayat and Rolf M. Fl{\"u}gel and Jorge Peries and Rodica Emanoil-Ravier},
  journal={Nucleic acids research},
  year={1988},
  volume={16 20},
  pages={9557-65}
}
Two forms of linear DNAs have been found in simian (SFV1) and human (HSRV) spumaviruses: a linear duplex unsensitive to nuclease S1 and a sensitive structure with a single-stranded gap. Two nuclease S1 sensitive sites, mapping at the same position for both viruses, have been identified in the gapped structure. Using different molecular subgenomic clones of HSRV as probes in Southern blot analysis, one S1 site was localized in the 3'LTR and the other near the middle of the molecule at about 6.5… CONTINUE READING