Evidence for a direct negative effect of estradiol at the level of the pituitary gland in sheep.

@article{Nett1990EvidenceFA,
  title={Evidence for a direct negative effect of estradiol at the level of the pituitary gland in sheep.},
  author={Terry M Nett and Jorge Antonio Flores and Fiorella Carnevali and J. P. Kile},
  journal={Biology of reproduction},
  year={1990},
  volume={43 4},
  pages={554-8}
}
The purpose of this experiment was to determine if pituitary stores of LH could be replenished by administration of GnRH when circulating concentrations of both progesterone and estradiol-17 beta (estradiol) were present at levels observed during late gestation. Ten ovariectomized (OVX) ewes were administered estradiol and progesterone via Silastic implants for 69 days. One group of 5 steroid-treated OVX ewes was given GnRH for an additional 42 days (250 ng once every 4 h). Steroid treatment… CONTINUE READING