Evidence for a concentration-dependent polymerization of a commercial human serum albumin.

@article{Zini1981EvidenceFA,
  title={Evidence for a concentration-dependent polymerization of a commercial human serum albumin.},
  author={Roland Zini and J{\'e}r{\^o}m{\'e} Barr{\'e} and Françoise Br{\'e}e and J. P. Tillement and Bernard S{\'e}bille},
  journal={Journal of chromatography},
  year={1981},
  volume={216},
  pages={
          191-8
        }
}
Polymerization of a commercial human serum albumin (Sigma A-1887) was investigated by two different techniques, high-performance liquid chromatography and gel electrophoresis. The chromatographic technique was based on the frontal analysis principle using a column which excludes polymers but retains monomers. The results allowed the determination of the monomer--polymer affinity constant, X = 526 +/- 100. The electrophoresis technique was performed with a polyacrylamide gel containing sodium… CONTINUE READING