Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells

@inproceedings{Boomsma2014EvidenceFT,
  title={Evidence for Transfer of Membranes from Mesenchymal Stem Cells to HL-1 Cardiac Cells},
  author={Robert A. Boomsma and David L. Geenen},
  booktitle={Stem cells international},
  year={2014}
}
This study examined the interaction of mouse bone marrow mesenchymal stem cells (MSC) with cardiac HL-1 cells during coculture by fluorescent dye labeling and then flow cytometry. MSC were layered onto confluent HL-1 cell cultures in a 1 : 4 ratio. MSC gained gap junction permeant calcein from HL-1 cells after 4 hours which was partially reduced by oleamide. After 20 hours, 99% MSC gained calcein, unaffected by oleamide. Double-labeling HL-1 cells with calcein and the membrane dye DiO resulted… CONTINUE READING
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