Evidence for Mechanosensitive Channel Activity of Tentonin 3/TMEM150C

@article{Hong2017EvidenceFM,
  title={Evidence for Mechanosensitive Channel Activity of Tentonin 3/TMEM150C},
  author={Gyu-Sang Hong and Byeongjun Lee and U. Oh},
  journal={Neuron},
  year={2017},
  volume={94},
  pages={271-273.e2}
}
Mechanosensation is essential for various physiological processes, and it is mediated by mechanotransduction channels. Recently, we reported that TMEM150C/Tentonin 3 (TTN3) confers mechanically activated currents with slow inactivation kinetics in several cell types, including dorsal root ganglion neurons (Hong et al., 2016). The accompanying Matters Arising by Dubin, Murthy, and colleagues confirms that naive heterologous cells demonstrate a mechanically activated current, but finds that this… Expand
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Endogenous Piezo1 Can Confound Mechanically Activated Channel Identification and Characterization
TLDR
Strikingly, these MA currents were not detected in cells in which endogenous Piezo1 was CRISPR/Cas9 inactivated, highlighting the importance of choosing cells lacking endogenous MA channels to assay the mechanotransduction properties of various proteins. Expand
Heterologous Expression of the Piezo1-ASIC1 Chimera Induces Mechanosensitive Currents with Properties Distinct from Piezo1
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The chimera results are discussed, potential interpretations are considered, and potential interpretations in light of the Matters Arising from Dubin et al. (2017), published concurrently in this issue of Neuron. Expand
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