Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation

  title={Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation},
  author={Paul Jr Wentworth and Jonathan E Mcdunn and Anita D. Wentworth and Cindy Takeuchi and Jorge J. Nieva and Teresa M. Jones and Cristina Bautista and Julie M. Ruedi and Abel Gutierrez and Kim D. Janda and Bernard M. Babior and Albert Prof Dr Eschenmoser and Richard A. Lerner},
  pages={2195 - 2199}
Recently, we showed that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen (1O2*) and water. Here, we show that this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2 production by antibodies alone was found to be not sufficient for bacterial killing. Our studies suggested that the antibody-catalyzed water-oxidation pathway produced an additional molecular species with a chemical signature… 

Ozone production by amino acids contributes to killing of bacteria

This study discovered that 4 amino acids themselves were able to catalyze the production of an oxidant with the chemical signature of ozone from singlet oxygen in the water-oxidation pathway, at comparable level to antibodies.

Evidence for the production of trioxygen species during antibody-catalyzed chemical modification of antigens

It is reported that the antibody-catalyzed water-oxidation process is capable of regioselectively converting antibody-bound benzoic acid into para-hydroxy benzosic acid as well as regioseslectively hydroxylating the 4-position of the phenyl ring of a single tryptophan residue located in the antibody molecule.

Effect of ozone produced from antibody-catalyzed water oxidation on pathogenesis of atherosclerosis.

Results showed that the production of O3, rather than H2O2, might be involved in the pathogenesis of atherosclerosis through the antibody-catalyzed water oxidation pathway, and suggested that the accumulation of all intracellular lipids was significantly inhibited by vinylbenzoic acid, and only slightly affected by catalase.

The antibody-catalyzed water oxidation pathway--a new chemical arm to immune defense?

Endogenous Generation of Singlet Oxygen and Ozone in Human and Animal Tissues: Mechanisms, Biological Significance, and Influence of Dietary Components

  • A. Onyango
  • Biology
    Oxidative medicine and cellular longevity
  • 2016
The potential pathways of formation of singlet oxygen and ozone in vivo are reviewed and some new pathways are proposed, as well as examples of how dietary factors may promote or inhibit their generation and activity.

Role of Nox2 in elimination of microorganisms

Critical review of the Nox2-dependent cellular processes that potentially contribute to bacterial killing and degradation and to indicate possible targets of pharmacological interventions are provided.

Do neutrophils produce ozone? An appraisal of current evidence

It is concluded that the evidence for ozone production by neutrophils is not convincing and studies using the same probes to implicate ozone in other biological processes should also be interpreted with caution.

Antimicrobial reactive oxygen and nitrogen species: concepts and controversies

  • F. Fang
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    Nature Reviews Microbiology
  • 2004
A review of the regulation, generation and actions of these molecular mediators, as well as their roles in resisting infection, updates the reader on these concepts and the topical questions in the field.



Antibody Catalysis of the Oxidation of Water

It is shown that antibodies can generate hydrogen peroxide from singlet molecular oxygen (1O2*), and the electron source for a quasi-unlimited generation of H2O2 is identified, suggesting a protective function of immunoglobulins against 1O2*, and raising the question of whether the need to detoxify 1O 2* has played a decisive role in the evolution of the immunoglOBulin fold.

Antibody-targeted Photolysis of Bacteria In Vivo

Irradiation resulted in a greater than 75% decrease in the number of viable bacteria at sites treated with a specific conjugate, whereas normal bacterial growth was observed in animals that were untreated or treating with a nonspecific conjugates.

Antibodies have the intrinsic capacity to destroy antigens.

It is reported that the immune system seems to have a previously unrecognized chemical potential intrinsic to the antibody molecule itself, thereby potentially aligning recognition and killing within the same molecule.

Kinetics and mechanism of the reaction between ozone and hydrogen peroxide in aqueous solutions

A kinetic model has been developed, taking into account both decomposition of ozone molecules and interactions between ozone and hydrogen peroxide for formation of hydroxyl radical and subsequent

Quantitative determination of aqueous-phase ozone by chemiluminescence using indigo-5,5'-disulfonate.

Indigo-5,5'-disulfonate (IDS) was found to be an efficient reagent for the determination of ozone by chemiluminescence (CL); hence it was applied to the continuous measurements of dissolved ozone

The gas phase reaction of singlet dioxygen with water: A water-catalyzed mechanism

The hydrogen polyoxides (H2O3 and H2O4) formed in these mechanisms and their decomposition product polyoxide radicals (HO2, HO3) may play a role in combustion, explosions, atmospheric chemistry, and the radiation chemistry in aqueous systems.

Increased Escherichia coli Phagocytosis in Neutrophils That Have Transmigrated across a Cultured Intestinal Epithelium

The phagocytic capacity of the PMNs is augmented after transepithelial migration, with a dramatic increase in the level of CD11b/CD18 and preservation of the superoxide production.