Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation

@article{Wentworth2002EvidenceFA,
  title={Evidence for Antibody-Catalyzed Ozone Formation in Bacterial Killing and Inflammation},
  author={Paul Jr Wentworth and Jonathan E Mcdunn and Anita D. Wentworth and Cindy Takeuchi and Jorge J. Nieva and Teresa M. Jones and Cristina Bautista and Julie M. Ruedi and Abel Gutierrez and Kim D. Janda and Bernard M. Babior and Albert Prof Dr Eschenmoser and Richard A. Lerner},
  journal={Science},
  year={2002},
  volume={298},
  pages={2195 - 2199}
}
Recently, we showed that antibodies catalyze the generation of hydrogen peroxide (H2O2) from singlet molecular oxygen (1O2*) and water. Here, we show that this process can lead to efficient killing of bacteria, regardless of the antigen specificity of the antibody. H2O2 production by antibodies alone was found to be not sufficient for bacterial killing. Our studies suggested that the antibody-catalyzed water-oxidation pathway produced an additional molecular species with a chemical signature… 
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314 Citations

Ozone production by amino acids contributes to killing of bacteria

This study discovered that 4 amino acids themselves were able to catalyze the production of an oxidant with the chemical signature of ozone from singlet oxygen in the water-oxidation pathway, at comparable level to antibodies.

Evidence for the production of trioxygen species during antibody-catalyzed chemical modification of antigens

It is reported that the antibody-catalyzed water-oxidation process is capable of regioselectively converting antibody-bound benzoic acid into para-hydroxy benzosic acid as well as regioseslectively hydroxylating the 4-position of the phenyl ring of a single tryptophan residue located in the antibody molecule.

Effect of ozone produced from antibody-catalyzed water oxidation on pathogenesis of atherosclerosis.

Results showed that the production of O3, rather than H2O2, might be involved in the pathogenesis of atherosclerosis through the antibody-catalyzed water oxidation pathway, and suggested that the accumulation of all intracellular lipids was significantly inhibited by vinylbenzoic acid, and only slightly affected by catalase.

The antibody-catalyzed water oxidation pathway--a new chemical arm to immune defense?

Endogenous Generation of Singlet Oxygen and Ozone in Human and Animal Tissues: Mechanisms, Biological Significance, and Influence of Dietary Components

  • A. Onyango
  • Biology
    Oxidative medicine and cellular longevity
  • 2016
The potential pathways of formation of singlet oxygen and ozone in vivo are reviewed and some new pathways are proposed, as well as examples of how dietary factors may promote or inhibit their generation and activity.

Role of Nox2 in elimination of microorganisms

Critical review of the Nox2-dependent cellular processes that potentially contribute to bacterial killing and degradation and to indicate possible targets of pharmacological interventions are provided.

Do neutrophils produce ozone? An appraisal of current evidence

It is concluded that the evidence for ozone production by neutrophils is not convincing and studies using the same probes to implicate ozone in other biological processes should also be interpreted with caution.

Functional variability of antibodies upon oxidative processes.

Antimicrobial reactive oxygen and nitrogen species: concepts and controversies

  • F. Fang
  • Biology
    Nature Reviews Microbiology
  • 2004
A review of the regulation, generation and actions of these molecular mediators, as well as their roles in resisting infection, updates the reader on these concepts and the topical questions in the field.

Biophotonic hydrogen peroxide production by antibodies, T cells, and T-cell membranes.

...

References

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