Evidence against defective trans-Golgi acidification in cystic fibrosis.

  title={Evidence against defective trans-Golgi acidification in cystic fibrosis.},
  author={Olivier Seksek and Joachim Biwersi and Alan S. Verkman},
  journal={The Journal of biological chemistry},
  volume={271 26},
Defective organelle acidification has been proposed as a unifying hypothesis to explain the pleiotropic cellular abnormalities associated with cystic fibrosis. To test whether cystic fibrosis transmembrane conductance regulator (CFTR) participates in trans-Golgi pH regulation, intraluminal trans-Golgi pH was measured in stably transfected Swiss 3T3 fibroblasts (expressing CFTR or DeltaF508-CFTR) and CFTR-expressing and nonexpressing epithelial cells. trans-Golgi pH was measured by ratio-imaging… CONTINUE READING


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