Corpus ID: 83030391

Evidence That a RecQ Helicase Slows

  title={Evidence That a RecQ Helicase Slows},
  author={J. Y. Lee-Soety},
  journal={PLOS Biology},
7 Citations
Rad6–Bre1-mediated H2B ubiquitination regulates telomere replication by promoting telomere-end resection
The model that Rad6–Bre1–H2Bub1 cooperates with MRX to promote telomere-end resection and thus positively regulates both telomerase- and recombination-dependent telomeres replication is supported. Expand
ATLAS: An advanced PCR-method for routine visualization of telomere length in Saccharomyces cerevisiae.
A telomere PCR approach is further developed and refined into a robust method allowing direct visualisation of telomeres length differences in routine experiments with S. cerevisiae, and showing a strong correlation of results with data obtained by Southern blot hybridization. Expand
Two routes to senescence revealed by real-time analysis of telomerase-negative single lineages
This work uses a microfluidics-based live-cell imaging assay to investigate replicative senescence in individual Saccharomyces cerevisiae cell lineages following telomerase inactivation and characterize two mechanistically distinct routes to senescences. Expand
Length-dependent processing of telomeres in the absence of telomerase
Rad5, a DNA helicase/Ubiquitin ligase of the error-free branch of the DNA damage tolerance (DDT) pathway, associates with native telomeres and cooperates with HR in senescent cells, and it is proposed that DDT acts in a length-independent manner, whereas an HR-based repair using the sister chromatid as a template buffers precocious 5′-3′ resection at the shortest telomere. Expand
Genome-Wide Analysis to Identify Pathways Affecting Telomere-Initiated Senescence in Budding Yeast
In telomerase-deficient yeast cells, like equivalent mammalian cells, telomeres shorten over many generations until a period of senescence/crisis is reached, and a genome-wide analysis identifies genes that affect entry into and/or exit from telomere-initiatedsenescence and will be of interest to those studying telomerre biology, replicative senescences, cancer, and ageing. Expand
Telomere replication: poised but puzzling
The delayed telomeric C‐strand synthesis at late S/G2 phase – an additional regulatory step of telomere maintenance and the CST complex are studied. Expand
The RecQ helicase AtRECQ4A is required to remove inter-chromosomal telomeric connections that arise during meiotic recombination in Arabidopsis.
A hitherto unknown role for a member of the RECQ helicase family during meiosis that contributes to the maintenance of chromosome integrity is identified. Expand


Rad51-dependent DNA structures accumulate at damaged replication forks in sgs1 mutants defective in the yeast ortholog of BLM RecQ helicase.
It is suggested that, in sgs1 cells, the unscheduled accumulation of Rad51-dependent cruciform structures at damaged forks result from defective maturation of recombination-dependent intermediates that originate from the replication-related sister chromatid junctions. Expand
The Saccharomyces cerevisiae WRN homolog Sgs1p participates in telomere maintenance in cells lacking telomerase
It is shown that WRN co‐localizes with telomeric factors in telomerase‐independent immortalized human cells, and further that the budding yeast RecQ family helicase Sgs1p influences telomere metabolism in yeast cells lacking telomersase. Expand
Evidence that the S.cerevisiae Sgs1 protein facilitates recombinational repair of telomeres during senescence
It is found that the S-phase checkpoint function of SGS1p is dispensable for preventing rapid senescence, but that Sgs1p sequences required for homologous recombination, including the helicase domain and topoisomerase III interaction domain, are essential. Expand
Anatomy and Dynamics of DNA Replication Fork Movement in Yeast Telomeric Regions
It is shown that the telomeric replication fork pause associated with the terminal TG1-3 tracts begins ∼100 bp upstream of the telomersic repeat tract sequence, and pausing is relieved by the Rrm3p helicase. Expand
Telomere Length Homeostasis Is Achieved via a Switch between Telomerase- Extendible and -Nonextendible States
It is demonstrated that telomere length homeostasis is achieved via a switch between telomerase-extendible and -nonextendible states by taking a molecular snapshot of a single round of telomeres replication. Expand
Branch migrating sister chromatid junctions form at replication origins through Rad51/Rad52-independent mechanisms.
Using two-dimensional gel electrophoresis, replication-dependent X-shaped molecules that appear during origin activation, branch migrate, and distribute along the replicon are identified and generated by cells through a mechanism influenced by the rate of fork progression. Expand
Recombination-mediated lengthening of terminal telomeric repeats requires the Sgs1 DNA helicase
  • H. Cohen, D. Sinclair
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2001
It is reported that telomerase-negative (est2) yeast cells lacking SGS1 senesced more rapidly, experienced a higher rate of telomere erosion, and were delayed in the generation of survivors, demonstrating that type II telomeres are not necessary for normal growth in the absence of telomersase. Expand
Accelerated loss of telomeric repeats may not explain accelerated replicative decline of Werner syndrome cells
While accelerated loss of telomeric repeats could potentially explain the rapid decline in proliferation of WS cells, it is possible that WS cells exit the cell cycle via mechanisms that differ from those of replicatively senescent cells from control subjects. Expand
Break-induced replication and recombinational telomere elongation in yeast.
When a telomere becomes unprotected or if only one end of a chromosomal double-strand break succeeds in recombining with a template sequence, DNA can be repaired by a recombination-dependent DNAExpand
Genetic Analysis Connects SLX5 and SLX8 to the SUMO Pathway in Saccharomyces cerevisiae
Results presented here suggest that SLX5 and SLX8 are new components or regulators of the SUMO pathway and that SUMO modification might have a general role in transcriptional regulation as part of the TBP regulatory network. Expand