Evasion of host defense by in vivo-produced protoplast-like cells of the insect mycopathogen Beauveria bassiana

  title={Evasion of host defense by in vivo-produced protoplast-like cells of the insect mycopathogen Beauveria bassiana},
  author={Jacquelyn C. Pendland and S. Y. Hung and Drion G. Boucias},
  journal={Journal of Bacteriology},
  pages={5962 - 5969}
In vivo cells (hyphal bodies) of the hyphomycetous insect pathogen Beauveria bassiana collected from host Spodoptera exigua larval hemolymph were osmotically sensitive and lacked a well-defined cell wall. In light and electron microscope studies, a galactose-specific lectin purified from S. exigua hemolymph, concanavalin A (specific for alpha-mannose), and a polyclonal antibody to B. bassiana cell walls all bound to surfaces of in vitro-produced B. bassiana blastospores; however, none of these… 

Uptake of the fluorescent probe FM4-64 by hyphae and haemolymph-derived in vivo hyphal bodies of the entomopathogenic fungus Beauveria bassiana.

Results suggest active uptake by different developmental stages of B. bassiana, including haemolymph-derived cells that can evade the insect immune system.

In vivo development of the entomogeneous hyphomycetePaecilomyces farinosus in hostSpodoptera exigua (beet armyworm) larvae

Neither phagocytosis of the initially injected in vitro-produced blastospores nor nodule formation around hyphal cells later in the infection process was effective in stopping fungal growth.

Lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus Beauveria bassiana.

Data indicate robust and diverse production of carbohydrate epitopes on different developmental stages of fungal cells and provide evidence that surface carbohydrates are elaborated in infection-specific patterns.

Phagocytosis of lectin-opsonized fungal cells and endocytosis of the ligand by insect Spodoptera exigua granular hemocytes: an ultrastructural and immunocytochemical study

Phagocytosis of blastospores of the fungal entomopathogen Paecilomyces farinosus by granular hemocytes from larvae of Spodoptera exigua was studied and actin was concentrated in the pseudopodia of phagocytic granulocytes and may be directly associated with lectin receptor(s).

Could insect phagocytic avoidance by entomogenous fungi have evolved via selection against soil amoeboid predators?

The data suggest that the ability of the fungal insect pathogens M. anisopliae and B. bassiana to survive insect phagocytic haemocytes may be a consequence of adaptations that have evolved in order to avoid predation by soil amoebae.

Induction of Novel Proteins inManduca sextaandBlaberus giganteusas a Response to Fungal Challenge

The results suggest that holometabolous and hemimetabolous insects synthesize novel proteins in response to fungal challenge and that sites of synthesis may be in different cell types (fat bodies or epidermal cells).

Discovery of a new intravacuolar protein required for the autophagy, development and virulence of Beauveria bassiana

A vital role of VLP4 is unveiled in B. bassiana and many more PSPs revealed in the previous transcriptome are called attention for new insights into the interactions of fungal insect pathogens with insects, including putative secretory proteins involved in fungal invasion and virulence.

The Mosquito Melanization Response Is Implicated in Defense against the Entomopathogenic Fungus Beauveria bassiana

The mosquito melanization response retards significantly B. bassiana growth and dissemination, a finding that may be exploited to design transgenic fungi with more potent bio-control activities against mosquitoes.



The Role of Surface Components of the Entomopathogenic Fungus Entomophaga aulicae in the Cellular Immune Response of Galleria mellonella (Lepidoptera)

SUMMARY: Larvae of Galleria mellonella (Lepidoptera) displayed a strong cellular reaction to the walled-stage hyphal bodies of the entomopathogenic fungus Entomophaga aulicae (Entomophthorales). In

Ultrastructural localization of carbohydrate in cell walls of the entomogenous hyphomycete Nomuraea rileyi

Several probes were used in this ultrastructural study to localize polysaccharides in cell walls on conidial germ tubes, hyphal bodies, and mycelia of the entomogenous hyphomycete Nomuraea rileyi, and labelling patterns did not vary from one morphological stage to another.

Lectin binding characteristics of several entomogenous hyphomycetes: possible relationship to insect hemagglutinins

Fungal surface sugar residues may be important with respect to insect defense mechanisms against invading cells and render the fungal cells more susceptible to phagocytosis or other insectdefense mechanisms.

Intracellular multiplication of Paracoccidioides brasiliensis in macrophages: killing and restriction of multiplication by activated macrophages

Findings indicate that resident macrophages are permissive for intracellular multiplication of P. brasiliensis and that this could be a factor in pathogenicity.

The Fungal Cell Wall and Its Involvement in the Pathogenic Process in Insect Hosts

The in vivo development of a “typical” insect pathogenic deuteromycete is reviewed, with emphasis on the composition of the fungal surfaces and with the interplay between thefungal cells and the host cell recognition mechanisms.

The galactose binding lectin from the beet armyworm, Spodoptera exigua: distribution and site of synthesis.

Adaptability of the coccidian Coelotropha to parasitism.