Evaluation of ultra performance liquid chromatography. Part I. Possibilities and limitations.

@article{deVilliers2006EvaluationOU,
  title={Evaluation of ultra performance liquid chromatography. Part I. Possibilities and limitations.},
  author={Andr{\'e} de Villiers and François Lestremau and Roman Szucs and Sylvie G{\'e}l{\'e}bart and Frank David and P. A. T. Sandra},
  journal={Journal of chromatography. A},
  year={2006},
  volume={1127 1-2},
  pages={
          60-9
        }
}
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TLDR
An electrically driven constant-flow syringe pump capable of generating mobile-phase pressures as high as 9000 bar (130,000 psi) is described, used in conjunction with an exponential dilution method for the gradient separation of peptides from a tryptic digest on a 27-cm-long capillary packed with 1.0-micron particles.
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Fused-silica capillary columns packed with 1.0-microm nonporous C18 bonded particles are evaluated with isocratic ultrahigh-pressure liquid chromatography and retention factors for moderately retained compounds are observed to increase with column i.d., suggesting an increase in packing density.
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