Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology.

  title={Evaluation of two-dimensional gel electrophoresis-based proteome analysis technology.},
  author={Steven P Gygi and Garry L. Corthals and Yunwu Zhang and Y. J. Rochon and Ruedi Aebersold},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={97 17},
Proteome analysis is most commonly accomplished by a combination of two-dimensional gel electrophoresis (2DE) to separate and visualize proteins and mass spectrometry (MS) for protein identification. Although this technique is powerful, mature, and sensitive, questions remain concerning its ability to characterize all of the elements of a proteome. In the current study, more than 1,500 features were visualized by silver staining a narrow pH range (4.9-5. 7) 2D gel in which 0.5 mg of total… CONTINUE READING
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