Evaluation of two-dimensional difference gel electrophoresis for protein profiling. Soluble proteins of the marine bacterium Pirellula sp. strain 1.

@article{Gade2003EvaluationOT,
  title={Evaluation of two-dimensional difference gel electrophoresis for protein profiling. Soluble proteins of the marine bacterium Pirellula sp. strain 1.},
  author={D{\"o}rte Gade and J{\"u}rgen Thiermann and Dieter Markowsky and Ralf Rabus},
  journal={Journal of molecular microbiology and biotechnology},
  year={2003},
  volume={5 4},
  pages={240-51}
}
Two-dimensional gel electrophoresis (2DE) is a central tool of proteome research, since it allows separation of complex protein mixtures at highest resolution. Quantification of gene expression at the protein level requires sensitive visualization of protein spots over a wide linear range. Two-dimensional difference gel electrophoresis (2D DIGE) is a new fluorescent technique for protein labeling in 2DE gels. Proteins are labeled prior to electrophoresis with fluorescent CyDyes trade mark and… CONTINUE READING

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