Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner.

@article{Fraser2002EvaluationOT,
  title={Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner.},
  author={Paul D Fraser and Susanne Romer and Cathie A. Shipton and Philippa B Mills and Joy W. Kiano and Norihiko Misawa and Rachel G Drake and Wolfgang W. Schuch and Peter M. Bramley},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99 2},
  pages={
          1092-7
        }
}
Phytoene synthase from the bacterium Erwinia uredovora (crtB) has been overexpressed in tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig). Fruit-specific expression was achieved by using the tomato polygalacturonase promoter, and the CRTB protein was targeted to the chromoplast by the tomato phytoene synthase-1 transit sequence. Total fruit carotenoids of primary transformants (T(0)) were 2-4-fold higher than the controls, whereas phytoene, lycopene, beta-carotene, and lutein levels were… CONTINUE READING

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U.S. Patent 5,618,988

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