Evaluation of three polymerase chain reaction techniques for detection of Brucella DNA in peripheral human blood.

@article{Baddour2008EvaluationOT,
  title={Evaluation of three polymerase chain reaction techniques for detection of Brucella DNA in peripheral human blood.},
  author={Manal Mohammad Baddour and Dalal H Alkhalifa},
  journal={Canadian journal of microbiology},
  year={2008},
  volume={54 5},
  pages={
          352-7
        }
}
Brucellosis is a widespread zoonosis. Currently the diagnosis of this zoonosis is based on microbiological and serological laboratory tests. Polymerase chain reaction (PCR) has been used to detect DNA from Brucella. Different target genes, primer pairs, PCR techniques, and extraction procedures have previously been published for Brucella detection. But only a few of these primers have been used in human samples, and only one study has been carried out to compare sensitivity between them. In the… 
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The results suggest that serum and blood analysis by conventional and real time PCR is a convenient and safe method for rapid and accurate diagnosis of brucellosis.
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