Evaluation of three-dimensional cultured HepG2 cells in a nano culture plate system: an in vitro human model of acetaminophen hepatotoxicity.

@article{Aritomi2014EvaluationOT,
  title={Evaluation of three-dimensional cultured HepG2 cells in a nano culture plate system: an in vitro human model of acetaminophen hepatotoxicity.},
  author={Kohei Aritomi and Yoichi Ishitsuka and Yoshiro Tomishima and Daisuke Shimizu and Nazuki Abe and Tsuyoshi Shuto and Mitsuru Irikura and Hirofumi Kai and Tetsumi Irie},
  journal={Journal of pharmacological sciences},
  year={2014},
  volume={124 2},
  pages={
          218-29
        }
}
Overdoses of acetaminophen (paracetamol, N-acetyl-p-aminophenol; APAP) cause severe liver injury, yet there is no common or high throughput in vitro human APAP model. This study examined the characteristics and usefulness of HepG2 cells grown in a nano culture plate (NCP) system, a three-dimensional culture method, as an in vitro human model for APAP-induced hepatotoxicity. The NCP-cultured HepG2 cells showed higher expression of mRNA and protein levels of cytochrome P450 2E1, which metabolizes… CONTINUE READING

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