Evaluation of the repeatability of a crude adult indirect Ostertagia ostertagi ELISA and methods of expressing test results.

@article{Sanchez2002EvaluationOT,
  title={Evaluation of the repeatability of a crude adult indirect Ostertagia ostertagi ELISA and methods of expressing test results.},
  author={Javier Mauricio Giraldo Sanchez and Ian R. Dohoo and Fred Markham and Ken Leslie and Gary A. Conboy},
  journal={Veterinary parasitology},
  year={2002},
  volume={109 1-2},
  pages={75-90}
}
An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Ostertagia ostertagi using a crude adult worm antigen was evaluated using serum and milk samples from adult cows, as well as from bulk tank milk. Within and between plate repeatabilities were determined. In addition, the effects of factors such as antigen batch, freezing, preserving of the samples and somatic cell counts (SCCs) of the samples were evaluated. Raw optical densities (ODs) and normalized… CONTINUE READING
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